pPolI-WSN-NP vector (V015589)

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LOCUS       V015589                 4591 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015589
VERSION     V015589
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 4591)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..4591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      complement(262..345)
                     /label="hepatitis delta ribozyme"
                     /note="hepatitis delta ribozyme"
                     /regulatory_class="terminator"
     misc_feature    complement(346..390)
                     /label="3'NRC"
                     /note="3'NRC"
     CDS             391..1884
                     /gene="NP"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Influenza A virus (strain
                     A/Wilson-Smith/1933 H1N1). Accession#: P15682"
     misc_feature    complement(1888..1910)
                     /label="5'NRC"
                     /note="5'NRC"
     regulatory      complement(1911..2048)
                     /label="human PolI promoter"
                     /note="human PolI promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2195..2211)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2685..2789
                     /label="AmpR promoter"
     CDS             2790..3647
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3821..4409
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"