pVAX1-BMP2-7-(-) vector (V015588)

MSDS

Basic Vector Information

Vector Name:: pVAX1-BMP2-7-(-)

Antibiotic Resistance:: Kanamycin

Length:: 6649 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Feichtinger GA.

Promoter:: CMV

pVAX1-BMP2-7-(-) vector Vector Map

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pVAX1-BMP2-7-(-) vector Sequence

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LOCUS       V015588                 6649 bp    DNA     circular SYN 01-MAY-2019
DEFINITION  Exported.
ACCESSION   V015588
VERSION     V015588
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Feichtinger GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2019) Division of Oral Biology, University of
            Leeds, Wellcome Trust Brenner Building, Level 7, St. James
            University Hospital Campus, Leeds, West Yorkshire LS9 7TF, United
            Kingdom
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-JAN-2019) Division of Oral Biology, University of Leeds,
            Wellcome Trust Brenner Building, Level 7, St. James University
            Hospital Campus, Leeds, West Yorkshire LS9 7TF, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..6649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        664..682
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             770..1957
                     /gene="BMP2"
                     /label="Bone morphogenetic protein 2"
                     /note="Bone morphogenetic protein 2 from Homo sapiens.
                     Accession#: P12643"
     polyA_signal    2042..2266
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2439..3230
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    complement(3424..3648)
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3858..5150)
                     /gene="BMP7"
                     /label="Bone morphogenetic protein 7"
                     /note="Bone morphogenetic protein 7 from Homo sapiens.
                     Accession#: P18075"
     promoter        complement(5195..5213)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5258..5461)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(5462..5841)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5942..6530)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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