pSBo-3 vector (V015584)

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LOCUS       62056_19340        4081 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pSBo-3, complete sequence.
ACCESSION   MN436823
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    44..50
                     /label=SapI
                     /note="SapI"
     misc_feature    52..54
                     /label=Gamma
                     /note="Gamma"
     misc_feature    56..59
                     /label=A
                     /note="A"
     misc_feature    61..66
                     /label=BsaI
                     /note="BsaI"
     protein_bind    131..152
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        167..197
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(258..314)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(666..671)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    673..676
                     /label=F
                     /note="F"
     misc_feature    678..680
                     /label=Epsilon
                     /note="Epsilon"
     misc_feature    complement(682..688)
                     /label=SapI
                     /note="SapI"
     misc_feature    692..731
                     /label=UNSX
                     /note="UNSX"
     terminator      743..774
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     primer_bind     complement(802..821)
                     /label=VR
                     /note="VR"
     misc_feature    822..862
                     /label=BBa_B0062
                     /note="BBa_B0062"
     CDS             complement(876..1688)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1689..1836)
                     /label=BBa_P1007 promoter
                     /note="BBa_P1007 promoter"
                     /regulatory_class="promoter"
     CDS             complement(2049..2996)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(3044..3266)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3758..3852)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      3889..3948
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     3961..3980
                     /label=VF2
                     /note="VF2"
     terminator      complement(4008..4037)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    4059..4070
                     /label=STOP all frames
                     /note="STOP all frames"
     misc_feature    4071..4081
                     /label=BBa_B0043
                     /note="BBa_B0043"