pEA206 vector (V015564)

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LOCUS       62056_8680        7861 bp DNA     circular SYN 02-MAR-2020
DEFINITION  Cloning vector pEA206, complete sequence.
ACCESSION   MN161744
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Liu C, Lin L, Wang J.
  TITLE     Absolute quantification of real-time PCR data with stage signal 
            difference analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Liu C, Lin L, Wang J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2019) State Key Laboratory of Electroanalytical 
            Chemistry, Changchun Institute of Applied Chemistry, Renmin Street, 
            Changchun, Jilin 130000, China
REFERENCE   3  (bases 1 to 7861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2019) State Key Laboratory of Electroanalytical Chemistry, 
            Changchun Institute of Applied Chemistry, Renmin Street, Changchun, 
            Jilin 130000, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        847..1551
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1569..1589
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1587..2192
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     CDS             2214..4079
                     /codon_start=1
                     /transl_table=11
                     /product="PhyB-N"
                     /label=PhyB-N
                     /protein_id="QID75589.1"
                     /translation="MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSL
                     RPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAY
                     LSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDV
                     RSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTE
                     DPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHED
                     EHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRL
                     TQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGL
                     VVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRD
                     SPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLS
                     TDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDG
                     QRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKES"
     terminator      4117..4304
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             4337..4360
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             complement(4431..5102)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(5103..5204)
                     /label=TRP1 promoter
     terminator      5276..5323
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5420..5875
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5901..6005
                     /label=AmpR promoter
     CDS             6006..6863
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7037..7625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"