pSMXL8-LUC vector (V015560)

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LOCUS       62056_20015        6573 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector pSMXL8-LUC, complete sequence.
ACCESSION   MN728548
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..6573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..2000
                     /label=promoter of AtSMXL8
                     /note="promoter of AtSMXL8"
                     /regulatory_class="promoter"
     CDS             2008..3657
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      3678..3930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3939..3955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4429..4533
                     /label=AmpR promoter
     CDS             4534..5391
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5565..6153
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6441..6462
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6477..6507
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6515..6531
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6539..6555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"