pVER-YFP-lacI vector (V015559)

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LOCUS       62056_22540        4313 bp DNA     circular SYN 27-DEC-2020
DEFINITION  Cloning vector pVER-YFP-lacI, complete sequence.
ACCESSION   MT702634
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Tack DS, Tonner PD, Pressman A, Olson ND, Levy SF, Romantseva EF, 
            Alperovich N, Vasilyeva O, Ross D.
  TITLE     The genotype-phenotype landscape of an allosteric protein
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Tack DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2020) Cellular Engineering, National Institute of 
            Standards and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD 
            20899, USA
REFERENCE   3  (bases 1 to 4313)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUL-2020) Cellular Engineering, National Institute of Standards 
            and Technology, 100 Bureau Dr MS8313, Gaithersburg, MD 20899, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             101..913
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1005..1549
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      complement(1996..2112)
                     /label=araC terminator
                     /note="araC terminator"
                     /regulatory_class="terminator"
     CDS             complement(2137..3216)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(3245..3298)
                     /label=PlacI promoter
                     /note="PlacI promoter"
                     /regulatory_class="promoter"
     regulatory      complement(3303..3363)
                     /label=L3S2P21 terminator
                     /note="L3S2P21 terminator"
                     /regulatory_class="terminator"
     CDS             complement(3367..4083)
                     /label=EYFP
                     /note="enhanced YFP"
     misc_RNA        complement(4133..4183)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    complement(4187..4203)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4211..4239)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     regulatory      complement(4261..4313)
                     /label=L3S3P21 terminator
                     /note="L3S3P21 terminator"
                     /regulatory_class="terminator"