Basic Vector Information
- Vector Name:
- 35S-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4696 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Wang L.
35S-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
35S-GFP vector Sequence
LOCUS 62056_106 4696 bp DNA circular SYN 20-JAN-2020 DEFINITION Cloning vector 35S-GFP, complete sequence. ACCESSION MN728541 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4696) AUTHORS Wang L. TITLE Direct Submission JOURNAL Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing 100101, China REFERENCE 2 (bases 1 to 4696) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing 100101, China" FEATURES Location/Qualifiers source 1..4696 /mol_type="other DNA" /organism="synthetic DNA construct" terminator 89..341 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(359..375) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(383..399) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(407..437) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(452..473) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(761..1349) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1523..2380) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2381..2485) /label=AmpR promoter primer_bind 2959..2975 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3489..3834 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 3918..4634 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK"
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