p15A-HNS-GFP vector (V015542)

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LOCUS       62056_1860        3744 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector p15A-HNS-GFP, complete sequence.
ACCESSION   MN623123
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food 
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, 
            China"
FEATURES             Location/Qualifiers
     source          1..3744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     gene            737..1147
                     /gene="hns"
                     /label=hns
     CDS             737..1147
                     /codon_start=1
                     /transl_table=11
                     /gene="hns"
                     /product="histone-like nucleoid structuring protein"
                     /label=hns
                     /note="HNS"
                     /protein_id="QJR97840.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEES
                     AAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDENG
                     ETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     CDS             1166..1882
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      1909..1980
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1996..2023
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2185..2730)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2844..2938)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2962..3618)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3619..3721)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"