pSimple-tub+eGFP vector (V015533)

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LOCUS       62056_19805        6569 bp DNA     circular SYN 21-APR-2020
DEFINITION  Cloning vector pSimple-tub+eGFP, complete sequence.
ACCESSION   MN496120
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Zhang Y, Wang H, Yang R, Wang L, Yang G, Liu T.
  TITLE     Genetic Transformation of Tribonema minus, a Eukaryotic Filamentous 
            Oleaginous Yellow-Green Alga
  JOURNAL   Int J Mol Sci 21 (6), E2106 (2020)
  PUBMED    32204356
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Zhang Y, Wang H, Liu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2019) Qingdao Institude of Bioenergy and 
            Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling
            Road, Laoshan District, Qingdao, Shandong 266101, China
REFERENCE   3  (bases 1 to 6569)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci"; date: "2020"; volume: "21"; issue: "6"; pages: "E2106"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-SEP-2019) Qingdao Institude of Bioenergy and Bioprocess 
            Technology, Chinese Academy of Sciences, No. 189 Songling Road, 
            Laoshan District, Qingdao, Shandong 266101, China"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: DNAMAN v. 7.0.2.176
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      245..1650
                     /label=derived from Tribonema minus tubulin
                     /note="derived from Tribonema minus tubulin"
                     /regulatory_class="promoter"
     CDS             1651..2367
                     /label=EGFP
                     /note="enhanced GFP"
     primer_bind     complement(2948..2964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3003..3021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3028..3044)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3185..3613
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             3957..4748
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4769..5626
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5800..6388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"