pPB-B2-TARG-GE vector (V015531)

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LOCUS       62056_18350        8263 bp DNA     circular SYN 03-JAN-2021
DEFINITION  Targeting vector pPB-B2-TARG-GE, complete sequence.
ACCESSION   MT917127
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Kondrashov A, Mohd Yusof NAN., Hasan A, Goulding J, Kodagoda T, 
            Hoang DM, Vo NTN., Melarangi T, Dolatshad N, Gorelik J, Hill SJ, 
            Harding SE, Denning C.
  TITLE     CRISPR/Cas9-mediated generation and analysis of N terminus 
            polymorphic models of beta2AR in isogenic hPSC-derived 
            cardiomyocytes
  JOURNAL   Mol Ther Methods Clin Dev 20, 39-53 (2020)
  PUBMED    33335946
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Kondrashov A, Mohd Yusof NAN., Hasan A, Goulding J, Kodagoda T, Minh
            DH, Thi NV, Melarangi T, Dolatshad N, Gorelik J, Hill S, Harding SE,
            Denning C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev 20, 39-53 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-AUG-2020) School of Medicine, Division of Cancer "
FEATURES             Location/Qualifiers
     source          1..8263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            698..1731
                     /gene="adrB2"
                     /label=adrB2
                     /note="5' homology arm"
     mobile_element  1732..2059
                     /mobile_element_type="transposon"
                     /label=transposon
                     /note="PiggyBac 5' terminal repeat domain"
     protein_bind    2060..2084
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2179..2678
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        2690..2737
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2762..3355
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     CDS             3362..4354
                     /label=Delta-TK
                     /note="truncated herpes simplex virus thymidine kinase
                     (Salomon et al., 1995)"
     polyA_signal    4385..4609
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4695..4719)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     mobile_element  4720..4967
                     /mobile_element_type="transposon"
                     /label=transposon
                     /note="PiggyBac 3' terminal repeat domain"
     gene            4968..5999
                     /gene="adrB2"
                     /label=adrB2
                     /note="3' homology arm"
     primer_bind     complement(6022..6038)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6075..6093)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6114..6130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6138..6154)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6162..6192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6207..6228)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6516..7104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7278..8135)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8136..8240)
                     /label=AmpR promoter