pcDNA3-HA-OPRD1 vector (V015526)

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LOCUS       V015526                 6531 bp    DNA     circular SYN 24-MAY-2020
DEFINITION  Exported.
ACCESSION   V015526
VERSION     V015526
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Zhou M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2020) SIMM, Zuchongzi Rd 500, Shanghai 201110,
            China
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (09-JAN-2020) SIMM, Zuchongzi Rd 500, Shanghai 201110, China"
FEATURES             Location/Qualifiers
     source          1..6531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    889..894
                     /label="HindIII restriction site"
                     /note="HindIII restriction site"
     CDS             904..930
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             937..2052
                     /gene="Oprd1"
                     /label="Delta-type opioid receptor"
                     /note="Delta-type opioid receptor from Mus musculus.
                     Accession#: P32300"
     misc_feature    2056..2061
                     /label="XhoI restriction site"
                     /note="XhoI restriction site"
     promoter        complement(2083..2101)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2127..2351
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2397..2825
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2839..3169
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3236..4027
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4204..4337
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4374..4390)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4398..4414)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4422..4452)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4467..4488)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4776..5364)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5538..6395)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6396..6500)
                     /label="AmpR promoter"