Basic Vector Information
- Vector Name:
- pGLM91(P_TRE-DsRed(FF4))
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4700 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Frei T, Cella F, Tedeschi F, Gutierrez J
- Promoter:
- tight TRE
pGLM91(P_TRE-DsRed(FF4)) vector Map
pGLM91(P_TRE-DsRed(FF4)) vector Sequence
LOCUS 62056_11790 4700 bp DNA circular SYN 17-NOV-2020 DEFINITION Cloning vector pGLM91(P_TRE-DsRed(FF4)), complete sequence. ACCESSION MT891335 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4700) AUTHORS Frei T, Cella F, Tedeschi F, Gutierrez J, Stan GB, Khammash M, Siciliano V. TITLE Characterization and mitigation of gene expression burden in mammalian cells JOURNAL Nat Commun 11 (1), 4641 (2020) PUBMED 32934213 REFERENCE 2 (bases 1 to 4700) AUTHORS Frei T, Cella F, Tedeschi F, Gutierrez J, Stan G-B., Khammash M, Siciliano V. TITLE Direct Submission JOURNAL Submitted (15-AUG-2020) Synthetic and Systems Biology for Biomedicine, Italian Institute of Technology-IIT, Largo Barsanti e Martteucci 53, Naples, Campania 80125, Italy REFERENCE 3 (bases 1 to 4700) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4641" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (15-AUG-2020) Synthetic and Systems Biology for Biomedicine, Italian Institute of Technology-IIT, Largo Barsanti e Martteucci 53, Naples, Campania 80125, Italy" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4700 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 1..266 /note="misc_signal; INS" /regulatory_class="other" polyA_signal 283..507 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" misc_feature 529..553 /label=bidirectional promoter artefact /note="bidirectional promoter artefact" promoter 554..868 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" misc_feature 896..905 /label=Kozak sequence /note="Kozak sequence" ncRNA 1181..1195 /label=pre_miRNA /note="miR-30a loop" /ncRNA_class="pre_miRNA" ncRNA 1198..1219 /label=miRNA /note="miR-FF4" /ncRNA_class="miRNA" ncRNA 1223..1351 /label=3' miR-30a /note="sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)" misc_binding 1988..2009 /label=miR-FF4 binding site /bound_moiety="miR-FF4" /note="mir FF4 target" misc_binding 2010..2031 /label=miR-FF4 binding site /bound_moiety="miR-FF4" /note="mir FF4 target" misc_binding 2032..2053 /label=miR-FF4 binding site /bound_moiety="miR-FF4" /note="mir FF4 target" promoter complement(2091..2109) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" polyA_signal 2267..2388 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" regulatory 2478..2767 /note="misc_signal; INS" /regulatory_class="other" rep_origin complement(2855..3443) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3617..4474) /label=AmpR /note="beta-lactamase" promoter complement(4475..4579) /label=AmpR promoter
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