pGLM91(P_TRE-DsRed(FF4)) vector (V015519)

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LOCUS       62056_11790        4700 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector pGLM91(P_TRE-DsRed(FF4)), complete sequence.
ACCESSION   MT891335
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan GB, Khammash M, 
            Siciliano V.
  TITLE     Characterization and mitigation of gene expression burden in 
            mammalian cells
  JOURNAL   Nat Commun 11 (1), 4641 (2020)
  PUBMED    32934213
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan G-B., Khammash M, 
            Siciliano V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2020) Synthetic and Systems Biology for 
            Biomedicine, Italian Institute of Technology-IIT, Largo Barsanti e 
            Martteucci 53, Naples, Campania 80125, Italy
REFERENCE   3  (bases 1 to 4700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4641"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2020) Synthetic and Systems Biology for Biomedicine, Italian
            Institute of Technology-IIT, Largo Barsanti e Martteucci 53, Naples,
            Campania 80125, Italy"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..266
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     polyA_signal    283..507
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    529..553
                     /label=bidirectional promoter artefact
                     /note="bidirectional promoter artefact"
     promoter        554..868
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    896..905
                     /label=Kozak sequence
                     /note="Kozak sequence"
     ncRNA           1181..1195
                     /label=pre_miRNA
                     /note="miR-30a loop"
                     /ncRNA_class="pre_miRNA"
     ncRNA           1198..1219
                     /label=miRNA
                     /note="miR-FF4"
                     /ncRNA_class="miRNA"
     ncRNA           1223..1351
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     misc_binding    1988..2009
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     misc_binding    2010..2031
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     misc_binding    2032..2053
                     /label=miR-FF4 binding site
                     /bound_moiety="miR-FF4"
                     /note="mir FF4 target"
     promoter        complement(2091..2109)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2267..2388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2478..2767
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     rep_origin      complement(2855..3443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3617..4474)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4475..4579)
                     /label=AmpR promoter