pG20_FLAG_Hyg vector (V015516)

LOCUS       62056_11135        5545 bp DNA     circular SYN 03-NOV-2020
DEFINITION  Cloning vector pG20_FLAG_Hyg, complete sequence.
ACCESSION   MT896414
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Pratt AI, Knoblauch J, Kunz H-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in 
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Pratt AI, Kunz H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington 
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
REFERENCE   3  (bases 1 to 5545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2020) School of Biological Sciences, Washington State 
            University, 1150 NE College Ave #385, Pullman, WA 99163, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..375
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             395..1417
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    1652..1828
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(1836..1858)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(1985..2420)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             2711..3523
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     repeat_region   3559..3583
                     /label=TDNA RB
                     /note="TDNA RB"
     rep_origin      3697..4285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4376..4400)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4565..4813)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(4824..4847)
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    complement(4848..4925)
                     /label=MCS
                     /note="MCS"
     regulatory      complement(4932..5545)
                     /label=pUBQ10
                     /note="pUBQ10"
                     /regulatory_class="promoter"