pNRVL-caSAT1 vector (V015514)

LOCUS       62056_18220        5220 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Cloning vector pNRVL-caSAT1, complete sequence.
ACCESSION   MN989861
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1094..1591
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     gene            1592..2818
                     /gene="caSAT1"
                     /label=caSAT1
     CDS             join(1592..1601,2256..2818)
                     /codon_start=1
                     /transl_table=11
                     /gene="caSAT1"
                     /product="streptothricin acetyltransferase"
                     /label=caSAT1
                     /note="codon-optimized for Candida albicans"
                     /protein_id="QID92292.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     regulatory      2822..2951
                     /label=Candida albicans URA3 terminator
                     /note="Candida albicans URA3 terminator"
                     /regulatory_class="terminator"
     misc_feature    2979..2984
                     /label=BssHII site for insert ligation
                     /note="BssHII site for insert ligation"
     misc_feature    2992..2997
                     /label=XhoI site for insert ligation
                     /note="XhoI site for insert ligation"
     misc_feature    2998..3448
                     /note="site of recombination; CpNEUT5LDowndown from C. 
                     parapsilosis"
     misc_feature    3449..3454
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     promoter        complement(3462..3480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3485..3501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3614..4420
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4570..5158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"