pAAV-TRE-C-prM-E-NS1 vector (V015509)

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LOCUS       V015509                 7293 bp    DNA     circular SYN 30-NOV-2021
DEFINITION  Exported.
ACCESSION   V015509
VERSION     V015509
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W.
  TITLE     Anterograde transneuronal tracing and genetic control with
            engineered yellow fever vaccine YFV-17D
  JOURNAL   Nat Methods (2021) In press
   PUBMED   34824475
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Xu W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Neuroscience, UT Southwestern Medical
            Center, Dallas, TX 75229, USA
REFERENCE   3  (bases 1 to 7293)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Methods
            (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-AUG-2021) Neuroscience, UT Southwestern Medical Center, Dallas,
            TX 75229, USA"
FEATURES             Location/Qualifiers
     source          1..7293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     polyA_signal    complement(164..245)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        261..575
                     /label="tight TRE promoter"
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV
                     promoter"
     CDS             612..4001
                     /note="Genome polyprotein from Yellow fever virus (isolate
                     Peru/1899/1981). Accession#: P29165"
                     /label="Genome polyprotein"
     polyA_signal    4040..4516
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4556..4696
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      4771..5226
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5508..5612
                     /label="AmpR promoter"
     CDS             5613..6470
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6644..7232
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"