Basic Vector Information
- Vector Name:
- AbVec6W-hIgG1
- Antibiotic Resistance:
- Streptomycin
- Length:
- 7035 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Davis CW, Jackson KJL., McElroy AK, Halfmann P
- Promoter:
- EM7
AbVec6W-hIgG1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AbVec6W-hIgG1 vector Sequence
LOCUS 62056_266 7035 bp DNA circular SYN 17-JUN-2019
DEFINITION Cloning vector AbVec6W-hIgG1, complete sequence.
ACCESSION MK909077
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7035)
AUTHORS Davis CW, Jackson KJL., McElroy AK, Halfmann P, Huang J, Chennareddy
C, Piper AE, Leung Y, Albarino CG, Crozier I, Ellebedy AH, Sidney J,
Sette A, Yu T, Nielsen SCA., Goff AJ, Spiropoulou CF, Saphire EO,
Cavet G, Kawaoka Y, Mehta AK, Glass PJ, Boyd SD, Ahmed R.
TITLE Longitudinal Analysis of the Human B Cell Response to Ebola Virus
Infection
JOURNAL Cell 177 (6), 1566-1582 (2019)
PUBMED 31104840
REFERENCE 2 (bases 1 to 7035)
AUTHORS Davis CW, Ahmed R.
TITLE Direct Submission
JOURNAL Submitted (09-MAY-2019) Microbiology and Immunology, Emory
University, 1462 Clifton Rd, Atlanta, GA 30322, USA
REFERENCE 3 (bases 1 to 7035)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
date: "2019"; volume: "177"; issue: "6"; pages: "1566-1582"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-MAY-2019) Microbiology and Immunology, Emory University, 1462
Clifton Rd, Atlanta, GA 30322, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7035
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 384..587
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 818..836
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
CDS 919..975
/label=IgH signal sequence
/note="signal sequence from the mouse immunoglobulin heavy
chain variable region"
V_region 976..1341
/gene="IgG1"
/note="VH from Ebola virus glycoprotein-specific mAb
5.6.1A02"
CDS 1342..2331
/label=hlgG1-CH
/note="Human IgG1 heavy chain constant region"
misc_feature 2344..2931
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 3038..3086
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter complement(3257..3275)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3280..3296)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3364..3792
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3806..4135
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 4183..4230
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 4249..4644
/label=BSD
/note="blasticidin S deaminase"
polyA_signal 4805..4938
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(5132..5720)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5819..6607)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
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