pMYC20 vector (V015501)

LOCUS       62056_17680        7890 bp DNA     circular SYN 10-FEB-2021
DEFINITION  Cloning vector pMYC20, complete sequence.
ACCESSION   MT572317
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Gross S, Schnell B, Haack P, Auerbach D, Mueller R.
  TITLE     In vivo and in vitro reconstitution of unique key steps in 
            cystobactamid antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Gross S, Schnell B, Haack P, Auerbach D, Mueller R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUN-2020) Microbial Natural Products, Helmholtz 
            Institute for Pharmaceutical Research Saarland, Helmholtz Center for
            Infection Research, Saarland University Campus E8.1, Saarbruecken, 
            Saarland 66123, Germany
REFERENCE   3  (bases 1 to 7890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JUN-2020) Microbial Natural Products, Helmholtz Institute for 
            Pharmaceutical Research Saarland, Helmholtz Center for Infection 
            Research, Saarland University Campus E8.1, Saarbruecken, Saarland 
            66123, Germany"
FEATURES             Location/Qualifiers
     source          1..7890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..248
                     /label=URA3 promoter
     CDS             249..1049
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      1166..1711
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        2079..2181
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2182..2838
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     repeat_region   3052..3080
                     /label=from Himar1
                     /note="from Himar1"
     oriT            3230..3339
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             3372..3740
                     /label=traJ
                     /note="oriT-recognizing protein"
     misc_feature    3812..4315
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_feature    4593..6423
                     /label=mx8 int building block
                     /note="mx8 int building block"
     gene            4699..6423
                     /gene="int"
                     /label=int
     CDS             4699..6423
                     /codon_start=1
                     /transl_table=11
                     /gene="int"
                     /product="integrase"
                     /label=int
                     /protein_id="QRG42409.1"
                     /translation="MVASRSRSWRRFSRRRGRRLRPKRRPTRRQRPSWRGRQGGAVGNV
                     YRKKATRYLWMWWFDAAGARQYASTGEEDEVKARAVLKATEKRVAAEKDQGLGASGPVT
                     VEAYAAKWMEGRVTLRSVMDDRSRMQHHVLPSLGDKELGVLTRRHVLALVREVRAKGLA
                     PRTVRHVYALVRTMLSDAVVDGLLERSPCDLRERRGEIPAIRDADPRWRDSAVYSRAEV
                     VALITDERIPLERRVLYAVAFVGAVRIGEASALQVRDWDGTVNPLGKLWVTKTFNHKVG
                     EVYETTKTDTPRQMPVHPWLSTMLASWLAYGWARMYARTPRQDDLILPSPTGMHQRPQG
                     VLRRLHADLDLLGFRRRRTHDARRTFISLARADGADKYLLQWVTHGRRSDMVDAYTTPP
                     WDALCREVAKLKLELPKQEEETMACRVPSCDSGVTADAGKEKPQQVRELAGAGVARSTG
                     LEPVLLGSFYANPRQLPTKSEGLDAESAKLARTGAVDWHKPVTAPHPLAVVRQQMGVLK
                     SGAGRERKVLASQLVDASLMALERLAAALHRTHAADDRWSDCPACRAVADGESPSAT"
     misc_feature    6055..6083
                     /gene="int"
                     /label=attP site
                     /note="attP site"
     promoter        6456..6484
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             6532..7719
                     /label=TcR
                     /note="tetracycline efflux protein"
     regulatory      7841..7890
                     /label=tD2 terminator
                     /note="tD2 terminator"
                     /regulatory_class="terminator"