pAS35_DUT1_HO vector (V015499)

Download GeneBank File(.gb)

LOCUS       V015499                 5580 bp    DNA     circular SYN 10-NOV-2020
DEFINITION  Exported.
ACCESSION   V015499
VERSION     V015499
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Cromie G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2020) Dudley Lab, PNRI, 720 Broadway, Seattle, WA
            98027, USA
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2020) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 98027,
            USA"
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(527..1384)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1385..1489)
                     /label="AmpR promoter"
     primer_bind     1963..1979
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2615..3055
                     /gene="DUT1"
                     /label="Deoxyuridine 5'-triphosphate nucleotidohydrolase"
                     /note="Deoxyuridine 5'-triphosphate nucleotidohydrolase
                     from Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P33317"
     promoter        3354..3697
                     /label="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     terminator      4281..4468
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4943..4959)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4967..4983)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4991..5021)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5036..5057)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(join(5345..5580,1..353))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"