pSEVA221AID-T7RNAP-plasmid-pSEVA221AID-T7RNAP vector (V015493)

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LOCUS       V015493                 7825 bp    DNA     circular SYN 07-DEC-2019
DEFINITION  Exported.
ACCESSION   V015493
VERSION     V015493
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Alvarez B, Mencia M, de Lorenzo V, Fernandez LA.
  TITLE     In vivo mutagenesis of target DNA in E. coli chromosome with
            processive fusions of base deaminases and T7 RNA polymerase
            terminated by RNA-guided dCas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Alvarez B, Fernandez LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2019) Microbial Biotechnology, CNB-CSIC (Centro
            Nacional de Biotecnologia-CSIC), Darwin 3, Cantoblanco, MADRID,
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 7825)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-SEP-2019) Microbial Biotechnology, CNB-CSIC (Centro Nacional de
            Biotecnologia-CSIC), Darwin 3, Cantoblanco, MADRID, Madrid 28049,
            Spain"
FEATURES             Location/Qualifiers
     source          1..7825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5..99
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             231..1043
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            1213..1321
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(1385..2530)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(2923..3454)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     terminator      complement(3585..3671)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3717..4340)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        4356..4411
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             4448..4480
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             4481..5071
                     /label="AID"
                     /note="human activation-induced cytidine deaminase
                     (Dickerson et al., 2003)"
     misc_feature    5078..5161
                     /gene="AID-T7RNAP"
                     /label="Linker"
                     /note="Linker"
     CDS             5168..7816
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"