JS_segE_C vector (V015492)

LOCUS       V015492                 8644 bp    DNA     circular SYN 01-APR-2020
DEFINITION  Exported.
ACCESSION   V015492
VERSION     V015492
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Shin J.
  TITLE     Programming Escherichia coli to function as a digital display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Shin J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2020) Biomedical Engineering, Boston University,
            44 Cummington Mall, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 8644)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JAN-2020) Biomedical Engineering, Boston University, 44
            Cummington Mall, Boston, MA 02215, USA"
FEATURES             Location/Qualifiers
     source          1..8644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(297..842)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(934..1746)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1903..1984
                     /label="PSrpR"
                     /note="PSrpR"
                     /regulatory_class="promoter"
     regulatory      1985..2054
                     /label="PLux2"
                     /note="PLux2"
                     /regulatory_class="promoter"
     misc_feature    2055..2129
                     /label="RiboJ53"
                     /note="RiboJ53"
     regulatory      2130..2147
                     /label="P3"
                     /note="P3"
                     /regulatory_class="ribosome_binding_site"
     CDS             2148..2750
                     /codon_start=1
                     /transl_table=11
                     /product="PhlF"
                     /label="PhlF"
                     /protein_id="QIQ56099.1"
                     /translation="MARTPSRSSIGSLRSPHTHKAILTSTIEILKECGYSGLSIESVAR
                     RAGASKPTIYRWWTNKAALIAEVYENESEQVRKFPDLGSFKADLDFLLRNLWKVWRETI
                     CGEAFRCVIAEAQLDPATLTQLKDQFMERRREMPKKLVENAISNGELPKDTNRELLLDM
                     IFGFCWYRLLTEQLTVEQDIEEFTFLLINGVCPGTQR"
     regulatory      2751..2808
                     /label="ECK120033737"
                     /note="ECK120033737"
                     /regulatory_class="terminator"
     regulatory      2813..2875
                     /label="PHlyIIR2"
                     /note="PHlyIIR2"
                     /regulatory_class="promoter"
     protein_bind    2896..2914
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2950..3024
                     /label="RiboJ10"
                     /note="RiboJ10"
     regulatory      3025..3058
                     /label="S4"
                     /note="S4"
                     /regulatory_class="ribosome_binding_site"
     CDS             3059..3700
                     /codon_start=1
                     /transl_table=11
                     /product="SrpR"
                     /label="SrpR"
                     /protein_id="QIQ56100.1"
                     /translation="MARKTAAEAEETRQRIIDAALEVFVAQGVSDATLDQIARKAGVTR
                     GAVYWHFNGKLEVLQAVLASRQHPLELDFTPDLGIERSWEAVVVAMLDAVHSPQSKQFS
                     EILIYQGLDESGLIHNRMVQASDRFLQYIHQVLRHAVTQGELPINLDLQTSIGVFKGLI
                     TGLLYEGLRSKDQQAQIIKVALGSFWALLREPPRFLLCEEAQIKQVKSFE"
     regulatory      3701..3790
                     /label="ECK120029600"
                     /note="ECK120029600"
                     /regulatory_class="terminator"
     regulatory      3795..4020
                     /label="PCin"
                     /note="PCin"
                     /regulatory_class="promoter"
     misc_feature    4021..4095
                     /label="RiboJ51"
                     /note="RiboJ51"
     regulatory      4096..4104
                     /label="H1"
                     /note="H1"
                     /regulatory_class="ribosome_binding_site"
     CDS             4105..4740
                     /codon_start=1
                     /transl_table=11
                     /product="HlyIIR"
                     /label="HlyIIR"
                     /protein_id="QIQ56101.1"
                     /translation="MKYILFEVCEMGKSREQTMENILKAAKKKFGERGYEGTSIQEITK
                     EAKVNVAMASYYFNGKENLYYEVFKKYGLANELPNFLEKNQFNPINALREYLTVFTTHI
                     KENPEIGTLAYEEIIKESARLEKIKPYFIGSFEQLKEILQEGEKQGVFHFFSINHTIHW
                     ITSIVLFPKFKKFIDSLGPNETNDTNHEWMPEDLVSRIISALTDKPNI"
     regulatory      4741..4800
                     /label="ECK120010799"
                     /note="ECK120010799"
                     /regulatory_class="terminator"
     regulatory      4847..4900
                     /label="PLacI"
                     /note="PLacI"
                     /regulatory_class="promoter"
     regulatory      4901..4928
                     /label="a part of PLacI"
                     /note="a part of PLacI"
                     /regulatory_class="ribosome_binding_site"
     CDS             4929..6008
                     /label="lacI"
                     /note="lac repressor"
     regulatory      6012..6029
                     /regulatory_class="ribosome_binding_site"
     CDS             6030..6650
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      6654..6908
                     /regulatory_class="terminator"
     regulatory      6948..6975
                     /regulatory_class="ribosome_binding_site"
     CDS             6976..7725
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     regulatory      7729..7756
                     /regulatory_class="ribosome_binding_site"
     CDS             7757..8512
                     /codon_start=1
                     /transl_table=11
                     /product="CinR"
                     /label="CinR"
                     /protein_id="QIQ56105.1"
                     /translation="MIENTYSEKFESAFEQIKAAANVDAAIRILQAEYNLDFVTYHLAQ
                     TIASKIDSPFVRTTYPDAWVSRYLLNCYVKVDPIIKQGFERQLPFDWSEVEPTPEAYAM
                     LVDAQKHGIDDNGYSIPVADKAQRRALLSLNAHIPADEWTELVRRCRNEWIEIAHLIHR
                     KAVYELHGENDPVPALSPREIECLHWTALGKDYKDISVILGISEHTTRDYLKTARFRLG
                     CTTISAAASRAVQLRIINPYRIRMTRRNW"
     terminator      8524..8595
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8611..8638
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"