RS511_ErbB-RASER1C-OFP vector (V015478)

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LOCUS       V015478                 9820 bp    DNA     circular SYN 18-MAY-2019
DEFINITION  Exported.
ACCESSION   V015478
VERSION     V015478
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Chung HK, Zou X, Bajar BT, Brand VR, Huo Y, Alcudia JF, Ferrell JE
            Jr., Lin MZ.
  TITLE     A compact synthetic pathway rewires cancer signaling to therapeutic
            effector release
  JOURNAL   Science 364 (6439) (2019)
   PUBMED   31048459
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Chung HK, Lin MZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2019) Bioengineering, Stanford University, 269
            Campus Drive, CCRS2100, Stanford, CA 94305, United States
REFERENCE   3  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science 364
            (6439) (2019)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-APR-2019) Bioengineering, Stanford University, 269 Campus Drive,
            CCRS2100, Stanford, CA 94305, United States"
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      12..564
                     /regulatory_class="promoter"
     misc_feature    67..354
                     /label="CAG_enhancer"
                     /note="CAG_enhancer"
     misc_feature    521..541
                     /label="CMV_fwd_primer"
                     /note="CMV_fwd_primer"
     LTR             584..764
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1435..1668
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1853..1897
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2046..2087
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2195..2312
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2371..2684
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das
                     et al., 1988)"
     primer_bind     2701..2717
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2759..2792
                     /label="loxP"
                     /note="loxP"
     enhancer        2908..3211
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3212..3415
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     3416..3465
                     /label="p1852 NheI kozak TM"
                     /note="p1852 NheI kozak TM"
     misc_feature    3448..3449
                     /label="nonstandard type: Editing History Replacement; GGT"
     CDS             3612..3638
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3864..4373
                     /label="CIBN"
                     /note="N-terminal portion of the CIB1 transcription factor
                     from Arabidopsis thaliana"
     misc_feature    complement(4374..4403)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1853"
     primer_bind     4389..4425
                     /label="p1727"
                     /note="p1727"
     misc_feature    4428..4760
                     /label="nonstandard type: SH2; VAV1"
     misc_feature    4818..4835
                     /label="nonstandard type: polylinker; L6"
     misc_feature    4872..4904
                     /label="nonstandard type: Substrate; substrate 4"
     misc_feature    4874..4904
                     /label="nonstandard type: Editing History Replacement;
                     AGATGTCGTGCCATGCTCAATGGGCTCG"
     CDS             4914..5564
                     /label="mKO2"
                     /note="monomeric Kusabira-Orange 2 fluorescent protein"
     misc_feature    5577..5618
                     /label="nonstandard type: epitope; V5"
     primer_bind     5577..5618
                     /label="p1291"
                     /note="p1291"
     misc_feature    5620..5690
                     /label="nonstandard type: Editing History Replacement;
                     AAAGCGGCCGCGTCGACGGGCCCGCGGAATTCCGCCCCCCCC
                     CCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGT
                     GTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGC
                     CCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCC
                     AAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTT
                     GAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGA
                     CAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAA
                     CCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAA
                     GCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGA
                     TCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTA
                     GGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCC
                     ACAACC"
     misc_feature    5628..5690
                     /note="T2A peptide from Thosea asigna virus capsid protein"
     misc_feature    complement(5637..5690)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1869 T2A
                     rev"
     primer_bind     5646..5705
                     /label="p1867 T2A PTB"
                     /note="p1867 T2A PTB"
     misc_feature    complement(5717..5753)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1856 SmaI
                     PTB rev"
     misc_feature    6054..6074
                     /label="HIF1a"
                     /note="HIF1a"
     CDS             6252..6275
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    6312..6890
                     /label="nonstandard type: Protease; NS3Tprotease"
     misc_feature    6471..6473
                     /label="nonstandard type: Editing History Replacement; GCA"
     primer_bind     6638..6670
                     /label="p1278"
                     /note="p1278"
     misc_feature    complement(6656..6686)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1502 MreI
                     rev"
     protein_bind    complement(6921..6954)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7010..7598
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(7601..7617)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    7783..7804
                     /label="U3PPT"
                     /note="U3PPT"
     misc_feature    7783..7798
                     /label="cPPT"
                     /note="cPPT"
     misc_feature    7840..8020
                     /label="truncHIV-1_3_LTR"
                     /note="truncHIV-1_3_LTR"
     rep_origin      complement(8082..8670)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8844..9701)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9702..9806)
                     /label="AmpR promoter"