pEVS170 vector (V015477)

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LOCUS       V015477                 5314 bp    DNA     circular SYN 13-FEB-2019
DEFINITION  Exported.
ACCESSION   V015477
VERSION     V015477
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Stoudenmire JL, Fidopiastis PM, Black M, Stabb EV.
  TITLE     Mutagenesis of Vibrio fischeri and other marine bacteria using
            hyperactive mini-Tn5 derivatives
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Stoudenmire JL, Fidopiastis PM, Black M, Stabb EV.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2018) Microbiology, University of Georgia, 120
            Cedar Street, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 5314)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2018) Microbiology, University of Georgia, 120 Cedar Street,
            Athens, GA 30602, USA"
FEATURES             Location/Qualifiers
     source          1..5314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..19
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     regulatory      complement(337..361)
                     /regulatory_class="terminator"
     CDS             complement(507..1241)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     regulatory      complement(1249..1256)
                     /regulatory_class="ribosome_binding_site"
     rep_origin      1560..1948
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1968..1992
                     /regulatory_class="terminator"
     primer_bind     2076..2092
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(2107..2125)
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             2259..3071
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(3418..3527)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(3802..5229)
                     /label="Tn5 transposase"
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"