Basic Vector Information
- Vector Name:
- 3-waxy-guide-CR3
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3700 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Gao H.
3-waxy-guide-CR3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
3-waxy-guide-CR3 vector Sequence
LOCUS 62056_96 3700 bp DNA circular SYN 19-MAR-2020 DEFINITION Cloning vector 3-waxy-guide-CR3, complete sequence. ACCESSION MN294715 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3700) AUTHORS Gao H. TITLE Direct Submission JOURNAL Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA REFERENCE 2 (bases 1 to 3700) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA" COMMENT ##Assembly-Data-START## Assembly Method :: Vector NTI v. 10 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3700 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 15..114 /label=attL1 /note="recombination site for the Gateway(R) LR reaction" regulatory 198..1197 /regulatory_class="promoter" misc_RNA 1217..1292 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" protein_bind complement(1364..1459) /label=attL3 /note="recombination site for the Gateway(R) LR reaction" promoter complement(1501..1519) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1524..1540) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 1653..2459 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 2609..3197 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(3527..3554) /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene"
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