Basic Vector Information
- Vector Name:
- pTrichoGate-8
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3476 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Nogueira-Lopez G.
pTrichoGate-8 vector Map
pTrichoGate-8 vector Sequence
LOCUS 62056_21545 3476 bp DNA circular SYN 28-AUG-2019 DEFINITION Cloning vector pTrichoGate-8, complete sequence. ACCESSION MN007112 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3476) AUTHORS Nogueira-Lopez G. TITLE Direct Submission JOURNAL Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, Christchurch, Canterbury 7647, New Zealand REFERENCE 2 (bases 1 to 3476) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, Christchurch, Canterbury 7647, New Zealand" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3476 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 27..743 /label=EGFP /note="enhanced GFP" misc_feature 744..780 /label=flexible linker GSAGSAAGSGEF /note="flexible linker GSAGSAAGSGEF" primer_bind complement(841..857) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(865..881) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(889..919) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(934..955) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1243..1831) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2005..2862) /label=AmpR /note="beta-lactamase" promoter complement(2863..2967) /label=AmpR promoter primer_bind 3441..3457 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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