pJTFlag vector (V015452)

LOCUS       62056_13230        5299 bp DNA     circular SYN 15-APR-2020
DEFINITION  Cloning vector pJTFlag, complete sequence.
ACCESSION   MN534898
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Juarez-Rodriguez MD, Torres-Escobar A, Ganta R.
  TITLE     ech_0660 and ech_0665 mutations in Ehrlichia chaffeensis cause 
            transcription changes in response to host-imposed zinc or iron 
            starvation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Juarez-Rodriguez MD, Torres-Escobar A, Ganta R.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2019) Department of Diagnostic 
            Medicine/Pathobiology, Kansas State University, 1800 Denison Ave, 
            Manhattan, KS 66506, USA
REFERENCE   3  (bases 1 to 5299)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-SEP-2019) Department of Diagnostic Medicine/Pathobiology, Kansas
            State University, 1800 Denison Ave, Manhattan, KS 66506, USA"
FEATURES             Location/Qualifiers
     source          1..5299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1497..2085
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2271..2413)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3481..3502)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4598..4675)
                     /label=lacI promoter
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    5069..5106
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     CDS             5113..5136
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5143..5160
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      5227..5274
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"