Integration-module-12-sequence vector (V015446)

LOCUS       V015446                 8403 bp    DNA     circular SYN 29-JUL-2019
DEFINITION  Exported.
ACCESSION   V015446
VERSION     V015446
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Castillo-Hair S, Baerman E, Fujita M, Igoshin O, Tabor J.
  TITLE     Optogenetic control of Bacillus subtilis gene expression
  JOURNAL   Nat Commun 10 (1), 3099 (2019)
   PUBMED   31308373
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   Castillo-Hair SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAY-2019) Bioengineering, Rice University, 6100 Main
            St., Houston, TX 77005, USA
REFERENCE   3  (bases 1 to 8403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "3099"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-MAY-2019) Bioengineering, Rice University, 6100 Main St.,
            Houston, TX 77005, USA"
FEATURES             Location/Qualifiers
     source          1..8403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..634
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="alpha-amylase"
     CDS             complement(647..1360)
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     regulatory      complement(1361..1384)
                     /label="MF001"
                     /note="MF001"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(1385..1458)
                     /label="PrpsD"
                     /note="PrpsD"
                     /regulatory_class="promoter"
     regulatory      1400
                     /label="+1"
                     /note="+1"
                     /regulatory_class="other"
     regulatory      complement(1408..1413)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(1431..1436)
                     /regulatory_class="minus_35_signal"
     gene            1765..2517
                     /gene="spc"
                     /label="spc"
     CDS             1765..2517
                     /codon_start=1
                     /transl_table=11
                     /gene="spc"
                     /product="spectinomycin resistance protein"
                     /label="spc"
                     /protein_id="QDQ17191.1"
                     /translation="MNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNSDLDFL
                     VVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYG
                     EWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMD
                     SSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERILLAVR
                     SYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            2644..3669
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="alpha-amylase"
     CDS             5032..5763
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      complement(6574..7162)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7336..8193)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8194..8298)
                     /label="AmpR promoter"