Basic Vector Information
- Vector Name:
- AF-11-19
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9928 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Williams AE, Sanchez-Vargas I, Reid WR, Lin J
AF-11-19 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
AF-11-19 vector Sequence
LOCUS 62056_281 9928 bp DNA circular SYN 17-NOV-2020
DEFINITION Cloning vector AF-11-19, complete sequence.
ACCESSION MT926370
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9928)
AUTHORS Williams AE, Sanchez-Vargas I, Reid WR, Lin J, Franz AWE., Olson KE.
TITLE The Antiviral Small-Interfering RNA Pathway Induces Zika Virus
Resistance in Transgenic Aedes aegypti
JOURNAL Viruses 12 (11), E1231 (2020)
PUBMED 33142991
REFERENCE 2 (bases 1 to 9928)
AUTHORS Williams A, Reid W, Sanchez-Vargas I, Lin J, Franz AWE., Kenneth OE.
TITLE Direct Submission
JOURNAL Submitted (24-AUG-2020) Veterinary Pathobiology, University of
Missouri, Connaway Hall, Columbia, MO 65211, USA
REFERENCE 3 (bases 1 to 9928)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Viruses";
date: "2020"; volume: "12"; issue: "11"; pages: "E1231"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(24-AUG-2020) Veterinary Pathobiology, University of Missouri,
Connaway Hall, Columbia, MO 65211, USA"
COMMENT ##Assembly-Data-START##
Assembly Method :: plasmidSPAdes v. 3.12.0
Assembly Name :: AF-11-19
Coverage :: 2000
Sequencing Technology :: Illumina
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9928
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 106..127
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..172
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_feature 239..1476
/label=upstream homology arm
/note="upstream homology arm"
regulatory complement(1507..1748)
/label=simian virus 40 large T antigen terminator
/note="simian virus 40 large T antigen terminator"
/regulatory_class="terminator"
misc_feature 1783..2320
/label=ZIKV dsRNA trigger
/note="ZIKV dsRNA trigger"
misc_feature complement(2321..2392)
/label=sialokinin-1 intron
/note="sialokinin-1 intron"
misc_feature complement(2393..2930)
/label=ZIKV dsRNA trigger
/note="ZIKV dsRNA trigger"
regulatory complement(2937..4093)
/label=carboxypeptidase A promoter
/note="carboxypeptidase A promoter"
/regulatory_class="promoter"
protein_bind 4108..4207
/label=phage phi-C31 attP
/note="attachment site of phage phi-C31"
polyA_signal complement(4310..4431)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(4557..5273)
/label=ECFP
/note="enhanced CFP"
regulatory complement(5274..5545)
/label=3xP3 eyeless promoter
/note="3xP3 eyeless promoter"
/regulatory_class="promoter"
protein_bind complement(5607..5706)
/label=phage phi-C31 attP
/note="attachment site of phage phi-C31"
misc_feature 5723..7465
/label=downstream homology arm
/note="downstream homology arm"
promoter 7970..8074
/label=AmpR promoter
CDS 8075..8932
/label=AmpR
/note="beta-lactamase"
rep_origin 9106..9694
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.