Basic Vector Information
- Vector Name:
- pMM7-10-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9544 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Das SR, Maselko MB, Upadhyay A, Smanski MJ.
pMM7-10-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMM7-10-1 vector Sequence
LOCUS 62056_17165 9544 bp DNA circular SYN 05-AUG-2020 DEFINITION Cloning vector pMM7-10-1, complete sequence. ACCESSION MN630871 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9544) AUTHORS Das SR, Maselko MB, Upadhyay A, Smanski MJ. TITLE Batch cultivation of non-transgenic males suitable for juvenile release SIT programs JOURNAL Unpublished REFERENCE 2 (bases 1 to 9544) AUTHORS Das SR, Maselko MB, Upadhyay A, Smanski MJ. TITLE Direct Submission JOURNAL Submitted (30-OCT-2019) Biotechnology Institute, University of Minnesota, 1479 Gortner Ave, St Paul, MN 55108, USA REFERENCE 3 (bases 1 to 9544) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (30-OCT-2019) Biotechnology Institute, University of Minnesota, 1479 Gortner Ave, St Paul, MN 55108, USA" FEATURES Location/Qualifiers source 1..9544 /mol_type="other DNA" /organism="synthetic DNA construct" promoter complement(24..42) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(49..65) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin complement(206..661) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 688..792 /label=AmpR promoter CDS 793..1650 /label=AmpR /note="beta-lactamase" rep_origin 1824..2412 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2700..2721 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2736..2766 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2774..2790 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2798..2814 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2835..2853 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind complement(3072..3141) /label=attB /note="attB site for the phi-C31 integrase (Groth et al., 2000)" protein_bind complement(3685..3963) /label=tetracycline response element /note="contains seven copies of the tetracycline operator tetO" regulatory 3983..4135 /label=Hsp70 /note="Hsp70" /regulatory_class="promoter" intron 4146..4212 /label=Mhc intron /note="modified Drosophila myosin heavy chain intron 16 (Pfeiffer et al., 2010)" 5'UTR 4234..4254 /label=syn21 5'UTR /note="syn21 5'UTR" CDS 4255..4878 /label=TetR /note="tetracycline repressor TetR" CDS 5035..5268 /label=VP16 AD /note="transcriptional activation domain of herpes simplex virus protein VP16 (Triezenberg et al., 1988; Cousens et al., 1989)" intron 5356..5421 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 5551..5571 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 5996..6128 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 6396..8204 /label=Polyubiquitin /note="Promoter of polyubiqutin gene of Drosophila melanogaster" CDS 8229..8249 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" CDS 8433..9149 /label=EGFP /note="enhanced GFP" misc_feature 9153..9393 /label=terminator /note="terminator" promoter complement(9462..9480) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 9511..9529 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase"
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