pMM7-10-1 vector (V015439)

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LOCUS       62056_17165        9544 bp DNA     circular SYN 05-AUG-2020
DEFINITION  Cloning vector pMM7-10-1, complete sequence.
ACCESSION   MN630871
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Das SR, Maselko MB, Upadhyay A, Smanski MJ.
  TITLE     Batch cultivation of non-transgenic males suitable for juvenile 
            release SIT programs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Das SR, Maselko MB, Upadhyay A, Smanski MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2019) Biotechnology Institute, University of 
            Minnesota, 1479 Gortner Ave, St Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 9544)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-OCT-2019) Biotechnology Institute, University of Minnesota, 1479
            Gortner Ave, St Paul, MN 55108, USA"
FEATURES             Location/Qualifiers
     source          1..9544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(24..42)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(49..65)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(206..661)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        688..792
                     /label=AmpR promoter
     CDS             793..1650
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1824..2412
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2700..2721
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2736..2766
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2774..2790
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2798..2814
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2835..2853
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(3072..3141)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    complement(3685..3963)
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     regulatory      3983..4135
                     /label=Hsp70
                     /note="Hsp70"
                     /regulatory_class="promoter"
     intron          4146..4212
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     5'UTR           4234..4254
                     /label=syn21 5'UTR
                     /note="syn21 5'UTR"
     CDS             4255..4878
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             5035..5268
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     intron          5356..5421
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5551..5571
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5996..6128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6396..8204
                     /label=Polyubiquitin
                     /note="Promoter of polyubiqutin gene of Drosophila
                     melanogaster"
     CDS             8229..8249
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             8433..9149
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    9153..9393
                     /label=terminator
                     /note="terminator"
     promoter        complement(9462..9480)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9511..9529
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"