Basic Vector Information
- Vector Name:
- pSG-DV_BC
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6846 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Stach C, McCann M, O'Brien C, Le TS
- Promoter:
- UbC
pSG-DV_BC vector Map
pSG-DV_BC vector Sequence
LOCUS V015436 6846 bp DNA circular SYN 23-OCT-2019
DEFINITION Exported.
ACCESSION V015436
VERSION V015436
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6846)
AUTHORS Stach C, McCann M, O'Brien C, Le TS, Somia N, Chen X, Lee K, Fu HY,
Daoutidis P, Zhao L, Hu WS, Smanski MJ.
TITLE Model-driven engineering of N-linked glycosylation in Chinese
Hamster Ovary cells
JOURNAL ACS Synth Biol (2019) In press
PUBMED 31596566
REFERENCE 2 (bases 1 to 6846)
AUTHORS Stach CS.
TITLE Direct Submission
JOURNAL Submitted (30-APR-2019) Biochemistry, Molecular Biology, and
Biophysics; Chemical Engineering and Materials Science, University
of Minnesota, 1479 Gortner Ave, Suite 344, Saint Paul, Minnesota
55108, United States
REFERENCE 3 (bases 1 to 6846)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol (2019) In press"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-APR-2019) Biochemistry, Molecular Biology, and Biophysics;
Chemical Engineering and Materials Science, University of Minnesota,
1479 Gortner Ave, Suite 344, Saint Paul, Minnesota 55108, United
States"
FEATURES Location/Qualifiers
source 1..6846
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 21..54
/label="lox2272"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
compatible with each other, but incompatible with loxP or
loxN sites (Lee and Saito, 1988)."
protein_bind complement(55..88)
/label="FRT (minimal)"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
misc_feature 89..94
/label="BbsI site"
/note="BbsI site"
misc_feature 97..100
/label="Golden gate cloning scar"
/note="Golden gate cloning scar"
misc_feature 105..111
/label="AarI site"
/note="AarI site"
primer_bind 313..329
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 330..386
/label="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(399..415)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(423..439)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(447..477)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(492..513)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 594..600
/label="AarI site"
/note="AarI site"
misc_feature 605..608
/label="Golden gate cloning scar"
/note="Golden gate cloning scar"
misc_feature 611..616
/label="BbsI site"
/note="BbsI site"
promoter 622..1833
/label="UbC promoter"
/note="human ubiquitin C promoter"
CDS 1865..2284
/gene="bsr"
/label="Blasticidin-S deaminase"
/note="Blasticidin-S deaminase from Bacillus cereus.
Accession#: P33967"
polyA_signal 2334..2382
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
protein_bind 2383..2416
/label="lox5171"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are
compatible with each other, but incompatible with loxP or
loxN sites (Lee and Saito, 1988)."
misc_feature 2417..2450
/label="F3"
/note="F3"
misc_feature 2469..2507
/label="PhiC31 attP"
/note="PhiC31 attP"
misc_feature 2697
/label="mutation to remove SapI site"
/note="mutation to remove SapI site"
CDS complement(2833..3690)
/label="AmpR"
/note="beta-lactamase"
terminator 4223..4309
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4401..4428
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
rep_origin 4562..5150
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5330
/label="mutation to remove SapI site"
/note="mutation to remove SapI site"
misc_feature complement(5336..5476)
/label="bom"
/note="basis of mobility region from pBR322"
CDS complement(6773..6790)
/label="6xHis"
/note="6xHis affinity tag"
protein_bind 6811..6844
/label="attB"
/note="minimal attB site for the phi-C31 integrase (Groth
et al., 2000)"
This page is informational only.