AGG1399 vector (V015432)

MSDS

Basic Vector Information

Vector Name:: AGG1399

Antibiotic Resistance:: Ampicillin

Length:: 2813 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J

AGG1399 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AGG1399 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_311        2813 bp DNA     circular SYN 12-AUG-2020
DEFINITION  Cloning vector AGG1399, complete sequence.
ACCESSION   MT119950
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Cas13b-dependent and Cas13b-independent RNA knockdown of viral 
            sequences in mosquito cells following guide RNA expression
  JOURNAL   Commun Biol 3 (1), 413 (2020)
  PUBMED    32737398
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright 
            Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE   3  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
            Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    187..208
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      226..760
                     /label=U6-3
                     /note="U6-3"
                     /regulatory_class="promoter"
     misc_feature    762..795
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    796..831
                     /label=PspCas13b guide backbone
                     /note="PspCas13b guide backbone"
     regulatory      832..838
                     /label=U6 terminator
                     /note="U6 terminator"
                     /regulatory_class="terminator"
     promoter        985..1089
                     /label=AmpR promoter
     CDS             1090..1947
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2121..2709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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