pTTC-Hsap-WT vector (V015428)

LOCUS       62056_21630        6214 bp DNA     circular SYN 25-JAN-2021
DEFINITION  MCherry and eGFP expression vector pTTC-Hsap-WT, complete sequence.
ACCESSION   MN232103
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Boldyreva LV, Yarinich LA, Kozhevnikova EN, Ivankin AV, Lebedev MO, 
            Pindyurin AV.
  TITLE     Fine gene expression regulation by minor sequence variations 
            downstream of the polyadenylation signal
  JOURNAL   Mol Biol Rep (2021) In press
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Boldyreva LV, Ivankin AV, Kozhevnikova EN, Pindyurin AV.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2019) Department of the Regulation of Genetic 
            Processes, Institute of Molecular and Cellular Biology, Lavrentyev 
            ave. 8/2, Novosibirsk, Novosibirsk Region 630090, Russia
REFERENCE   3  (bases 1 to 6214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Rep (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2019) Department of the Regulation of Genetic Processes, 
            Institute of Molecular and Cellular Biology, Lavrentyev ave. 8/2, 
            Novosibirsk, Novosibirsk Region 630090, Russia"
FEATURES             Location/Qualifiers
     source          1..6214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    838..859
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        874..904
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    912..928
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     936..952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(989..1122)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1132..1836)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        complement(1913..1931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1976..2179)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2180..2483)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2558..3068
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3078..3794
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      3890..3906
                     /label=human neuropilin 1 (NRP1) polyA signal
                     /note="human neuropilin 1 (NRP1) polyA signal"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    3913..3979
                     /note="piggyBac transposon 3' terminal repeat sequence
                     together with the adjacent TTAA target site"
     promoter        complement(4026..4044)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4065..4081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4089..4105)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4113..4143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4158..4179)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4467..5055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5229..6086)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6087..6191)
                     /label=AmpR promoter