pmET32c vector (V015414)

LOCUS       V015414                 7425 bp    DNA     circular SYN 01-AUG-2019
DEFINITION  Exported.
ACCESSION   V015414
VERSION     V015414
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Soleymani S, Kalidari GA, Bassami MR, Dehghani H, Hashemi Tabar GR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2019) Pathobiology, Division of Biotechnology,
            Faculty of Veterinary Medicine, Ferdowsi University of Mashhad,
            Azadi Square, Mashhad, Razavi Khorasan 9177948974, Iran
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-JUN-2019) Pathobiology, Division of Biotechnology, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 9177948974, Iran"
FEATURES             Location/Qualifiers
     source          1..7425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    254..275
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             1050..1238
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1343..1485
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1671..2259)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2347..2375
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             2564..3094
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        3387..3464
                     /label="lacI promoter"
     CDS             3465..4544
                     /label="lacI"
                     /note="lac repressor"
     promoter        4698..4726
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4727..4751
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4773..4795
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4818..5141
                     /label="TrxA"
                     /note="E. coli thioredoxin"
     CDS             5166..5612
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             5613..5630
                     /label="6xHis"
                     /note="6xHis affinity tag"
     regulatory      5690..5847
                     /label="rrnB terminator"
                     /note="rrnB terminator"
                     /regulatory_class="terminator"
     terminator      complement(6180..6227)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(6294..6311)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(6324..7034)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      7082..7109
                     /label="lambda PR"
                     /note="lambda PR"
                     /regulatory_class="promoter"
     CDS             7138..7410
                     /gene="E"
                     /label="Lysis protein E"
                     /note="Lysis protein E from Enterobacteria phage phiX174.
                     Accession#: P03639"