3XFlag-SMXL6-no-EAR vector (V015413)

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LOCUS       62056_126        7289 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector 3XFlag-SMXL6-no-EAR, complete sequence.
ACCESSION   MN780594
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             448..513
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     protein_bind    514..538
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             541..3462
                     /codon_start=1
                     /transl_table=11
                     /product="AtSMXL6 no EAR"
                     /label=AtSMXL6 no EAR
                     /note="AtSMXL6 protein with EAR notif deletion"
                     /protein_id="QHH26575.1"
                     /translation="MPTPVTTARECLTEEAARALDDAVVVARRRSHAQTTSLHAVSALL
                     AMPSSILREVCVSRAARSVPYSSRLQFRALELCVGVSLDRLPSSKSPATEEDPPVSNSL
                     MAAIKRSQANQRRHPESYHLQQIHASNNGGGGCQTTVLKVELKYFILSILDDPIVNRVF
                     GEAGFRSSEIKLDVLHPPVTQLSSRFSRGRCPPLFLCNLPNSDPNREFPFSGSSGFDEN
                     SRRIGEVLGRKDKKNPLLIGNCANEALKTFTDSINSGKLGFLQMDISGLSLISIEKEIS
                     EILADGSKNEEEIRMKVDDLGRTVEQSGSKSGIVLNLGELKVLTSEANAALEILVSKLS
                     DLLKHESKQLSFIGCVSSNETYTKLIDRFPTIEKDWDLHVLPITASTKPSTQGVYPKSS
                     LMGSFVPFGGFFSSTSNFRVPLSSTVNQTLSRCHLCNEKYLQEVAAVLKAGSSLSLADK
                     CSEKLAPWLRAIETKEDKGITGSSKALDDANTSASQTAALQKKWDNICQSIHHTPAFPK
                     LGFQSVSPQFPVQTEKSVRTPTSYLETPKLLNPPISKPKPMEDLTASVTNRTVSLPLSC
                     VTTDFGLGVIYASKNQESKTTREKPMLVTLNSSLEHTYQKDFKSLREILSRKVAWQTEA
                     VNAISQIICGCKTDSTRRNQASGIWLALLGPDKVGKKKVAMTLSEVFFGGKVNYICVDF
                     GAEHCSLDDKFRGKTVVDYVTGELSRKPHSVVLLENVEKAEFPDQMRLSEAVSTGKIRD
                     LHGRVISMKNVIVVVTSGIAKDNATDHVIKPVKFPEEQVLSARSWKLQIKLGDATKFGV
                     NKRKYELETAQRAVKVQRSYVNETEFSPDHEAEDRDAWFDEFIEKVDGKVTFKPVDFDE
                     LAKNIQEKIGSHFERCFGSETHLELDKEVILQILAASWSSLSSGEEEGRTIVDQWMQTV
                     LARSFAEAKQKYGSNPMLGVKLVASSSGLASGVELPAKVDVIW"
     protein_bind    complement(3464..3488)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3753..3771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3778..3794)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3935..4390
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4468..4572
                     /label=AmpR promoter
     CDS             4573..5430
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5604..6192
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6480..6501
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6516..6546
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6554..6570
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6578..6594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6612..6630
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6803..7289
                     /label=CaMV35S(long)
                     /note="Cauliflower mosaic virus 35S promoter (long)"