Basic Vector Information
- Vector Name:
- pS238D.M
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7249 bp
- Type:
- Expression vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Calles B, Goni-Moreno A, de Lorenzo V.
- Promoter:
- Pm
pS238D.M vector Map
pS238D.M vector Sequence
LOCUS 62056_19135 7249 bp DNA circular SYN 26-NOV-2019 DEFINITION Expression vector pS238D.M, complete sequence. ACCESSION MN393461 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7249) AUTHORS Calles B, Goni-Moreno A, de Lorenzo V. TITLE Digitalizing heterologous gene expression in Gram-negative bacteria with a portable on/off module JOURNAL Unpublished REFERENCE 2 (bases 1 to 7249) AUTHORS Calles B, Goni-Moreno A, de Lorenzo V. TITLE Direct Submission JOURNAL Submitted (28-AUG-2019) Systems Biology Program, Centro Nacional de Biotecnologia, Darwin, 3, Cantoblanco, Madrid 28049, Spain REFERENCE 3 (bases 1 to 7249) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (28-AUG-2019) Systems Biology Program, Centro Nacional de Biotecnologia, Darwin, 3, Cantoblanco, Madrid 28049, Spain" FEATURES Location/Qualifiers source 1..7249 /mol_type="other DNA" /organism="synthetic DNA construct" terminator 5..99 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS 231..1043 /label=KanR /note="aminoglycoside phosphotransferase" oriT 1213..1321 /label=oriT /note="incP origin of transfer" CDS complement(1335..1994) /label=pBBR1 Rep /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" rep_origin complement(1995..2766) /direction=LEFT /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" terminator complement(2881..2967) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(2995..3957) /label=XylS /note="XylS regulator encoded by the Pseudomonas putida TOL plasmid pWWO" promoter 4857..4937 /label=Pm promoter /note="The bacterial Pm promoter is activated by XylS in the presence of benzoate or m-toluate (Marques et al., 1999)." regulatory 4972..4978 /regulatory_class="ribosome_binding_site" CDS 4990..6069 /label=lacI /note="lac repressor" CDS 6070..6087 /label=6xHis /note="6xHis affinity tag" CDS 6116..6826 /label=superfolder GFP /note="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" misc_feature 6830..6886 /label=MCS /note="pUC18/19 multiple cloning site" regulatory 6919..6948 /label=synthetic T500 terminator /note="synthetic T500 terminator" /regulatory_class="terminator" terminator complement(6955..6982) /label=T7Te terminator /note="phage T7 early transcription terminator" terminator complement(7022..7068) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" regulatory 7083..7191 /note="anti LacI sRNA; MicC-based" /regulatory_class="other" regulatory complement(7192..7249) /label=PA1/O4s /note="PA1/O4s" /regulatory_class="promoter"
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