AGG1533 vector (V015400)

MSDS

Basic Vector Information

Vector Name:: AGG1533

Antibiotic Resistance:: Ampicillin

Length:: 5646 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Xu X, Yang J, Harvey-Samuel T, Huang Y

Promoter:: lac UV5

AGG1533 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AGG1533 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_321        5646 bp DNA     circular SYN 25-APR-2020
DEFINITION  Cloning vector AGG1533, complete sequence.
ACCESSION   MN720705
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Xu X, Yang J, Harvey-Samuel T, Huang Y, Asad M, Chen W, He W, Yang 
            G, Alphey L, You M.
  TITLE     Identification and characterization of the vasa gene in the 
            diamondback moth, Plutella xylostella
  JOURNAL   Insect Biochem. Mol. Biol. (2020) In press
  PUBMED    32283279
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Xu X, Harvey-Samuel T, Alphey L, You M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2019) The Pirbright Insitute, Ash Road, Pirbright,
            Woking GU24 0NF, UK
REFERENCE   3  (bases 1 to 5646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect 
            Biochem. Mol. Biol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-NOV-2019) The Pirbright Insitute, Ash Road, Pirbright, Woking 
            GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..5646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      372..912
                     /label=Opie2
                     /note="Opie2"
                     /regulatory_class="promoter"
     intron          931..993
                     /note="Scraps"
     CDS             1021..2802
                     /label=PBase
                     /note="Native piggyBac transposase from Trichoplusia ni"
     polyA_signal    complement(2831..2952)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3454..3470)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3478..3508)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(3523..3544)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3835..4423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4597..5454)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5455..5559)
                     /label=AmpR promoter

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