pSEVA221TrxA-AIDT7RNAP vector (V015399)

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LOCUS       V015399                 8149 bp    DNA     circular SYN 06-OCT-2020
DEFINITION  Exported.
ACCESSION   V015399
VERSION     V015399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Alvarez B, Mencia M, de Lorenzo V, Fernandez LA.
  TITLE     In vivo diversification of target genomic sites using processive T7
            RNA polymerase-base deaminase fusions blocked by RNA-guided dCas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Alvarez B, Fernandez LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2020) Microbial Biotechnology, CNB-CSIC (Centro
            Nacional de Biotecnologia-CSIC), Darwin 3, Cantoblanco, Madrid,
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 8149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-JUL-2020) Microbial Biotechnology, CNB-CSIC (Centro Nacional de
            Biotecnologia-CSIC), Darwin 3, Cantoblanco, Madrid, Madrid 28049,
            Spain"
FEATURES             Location/Qualifiers
     source          1..8149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(32..118)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(164..787)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        803..858
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             898..1221
                     /label="HP-thioredoxin"
                     /note="His-Patch thioredoxin"
     CDS             1237..1251
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             1252..1842
                     /label="AID"
                     /note="human activation-induced cytidine deaminase
                     (Dickerson et al., 2003)"
     misc_feature    1849..1932
                     /gene="TrxA-AID-T7RNAP"
                     /label="Linker"
                     /note="Linker"
     CDS             1939..4587
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     terminator      4601..4695
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             4827..5639
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            5809..5917
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(5981..7126)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(7519..8050)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"