Basic Vector Information
- Vector Name:
- pDXTP
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 9939 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Sterner ZR, Rankin SA, Wlizla M, Choi JA
- Promoter:
- lac UV5
pDXTP vector Vector Map
pDXTP vector Sequence
LOCUS 62056_8650 9939 bp DNA circular SYN 26-JAN-2020
DEFINITION Cloning vector pDXTP, complete sequence.
ACCESSION MN044709
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9939)
AUTHORS Sterner ZR, Rankin SA, Wlizla M, Choi JA, Luedeke DM, Zorn AM,
Buchholz DR.
TITLE Novel vectors for functional interrogation of Xenopus ORFeome coding
sequences
JOURNAL Genesis 57 (10), e23329 (2019)
PUBMED 31403250
REFERENCE 2 (bases 1 to 9939)
AUTHORS Buchholz DR.
TITLE Direct Submission
JOURNAL Submitted (07-JUN-2019) Biological Sciences, University of
Cincinnati, 312 Clifton Ct., Cincinnati, OH 45221, USA
REFERENCE 3 (bases 1 to 9939)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genesis";
date: "2019"; volume: "57"; issue: "10"; pages: "e23329"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-JUN-2019) Biological Sciences, University of Cincinnati, 312
Clifton Ct., Cincinnati, OH 45221, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9939
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 22..55
/label=attB
/note="minimal attB site for the phi-C31 integrase (Groth
et
al., 2000)"
protein_bind 56..73
/label=I-SceI meganuclease binding site
/bound_moiety="I-SceI meganuclease"
protein_bind 74..273
/label=tol2 binding site
/bound_moiety="tol2"
/note="tol2 5' site, the minimal 200bp"
regulatory 274..919
/note="chicken HS4 extended (composed of HS4 core and HS4
3' 400 bp)"
/regulatory_class="insulator"
protein_bind 931..978
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
protein_bind 1001..1034
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
protein_bind 1089..1213
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 1238..1268
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 1322..1978
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 2323..2625
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(2669..2793)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS 2817..3560
/label=Tet-On(R) 3G
/note="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
regulatory 3578..4033
/label=SV40 polyadenylation sequence
/note="SV40 polyadenylation sequence"
/regulatory_class="other"
regulatory 4034..4679
/note="chicken HS4 extended (composed of HS4 core and HS4
3' 400 bp)"
/regulatory_class="insulator"
regulatory 4718..5031
/label=CRY promoter
/note="CRY promoter"
/regulatory_class="promoter"
CDS 5032..5745
/label=mgfp5
/note="GFP with folding enhancement mutations"
polyA_signal complement(5798..5932)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 6025..6042
/label=I-SceI meganuclease binding site
/bound_moiety="I-SceI meganuclease"
protein_bind 6109..6142
/label=lox2272
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
compatible with each other, but incompatible with loxP or
loxN sites (Lee and Saito, 1988)."
protein_bind 6165..6212
/label=flippase recombinase binding site
/bound_moiety="flippase recombinase"
/note="FRT3 site"
regulatory 6224..6869
/note="chicken HS4 extended (composed of HS4 core and HS4
3' 400 bp)"
/regulatory_class="insulator"
protein_bind 6870..7019
/label=tol2 binding site
/bound_moiety="tol2"
/note="tol2 3'site"
protein_bind complement(7020..7037)
/label=I-SceI meganuclease binding site
/bound_moiety="I-SceI meganuclease"
promoter complement(7064..7082)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(7100..7116)
/label=M13R
/note="M13R"
promoter complement(7148..7178)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7193..7214)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7502..8090)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8264..9121)
/label=AmpR
/note="beta-lactamase"
promoter complement(9122..9226)
/label=AmpR promoter
rep_origin complement(9304..9759)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 9880..9896
/label=pUC/M13F
/note="pUC/M13F"
promoter 9923..9939
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
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