pTrichoGate-19 vector (V015386)

LOCUS       62056_21500        5968 bp DNA     circular SYN 28-AUG-2019
DEFINITION  Cloning vector pTrichoGate-19, complete sequence.
ACCESSION   MN007123
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Nogueira-Lopez G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, 
            Lincoln, Christchurch, Canterbury 7647, New Zealand
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, 
            Christchurch, Canterbury 7647, New Zealand"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..2445
                     /gene="CbxR"
                     /label=CbxR
     regulatory      1..961
                     /gene="CbxR"
                     /label=succinate dehydrogenase promoter region (sdhP)
                     /note="succinate dehydrogenase promoter region (sdhP)"
                     /regulatory_class="promoter"
     CDS             join(962..1465,1536..1862)
                     /codon_start=1
                     /transl_table=11
                     /gene="CbxR"
                     /product="succinate dehydrogenase"
                     /label=CbxR
                     /note="sdh; carboxin resistance gene"
                     /protein_id="QEG99044.1"
                     /translation="MAALRSSTRILSSSTRAAFRPSAVFSRSMASVSETTEQQPRVKSF
                     QIYRWNPDTPTEKPRLQTYSIDLNKTGPMILDALIKIKNEQDPSLTFRRSCREGICGSC
                     AMNINGQNTLACLCRIPTESASDVKIYPLPHTYVVKDLVPDLTHFYKQYKSIQPYLQRD
                     TPAEDGKEYRQSKADRKKLDGLYECILCACCSTSCPSYWWNSEEYLGPAILLQSYRWLI
                     DSRDERTEARKSKLENSMSLYRCLTILNCTRACPKGLNPGKAIAEIKKQMSLGA"
     intron          1466..1535
                     /gene="CbxR"
     regulatory      1863..2445
                     /gene="CbxR"
                     /label=succinate dehydrogenase terminator region (sdhT)
                     /note="succinate dehydrogenase terminator region (sdhT)"
                     /regulatory_class="terminator"
     promoter        complement(2518..2536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2543..2559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2697..2996
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             3348..4139
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4349..4720
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      4861..5449
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5737..5758
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5773..5803
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5811..5827
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5835..5851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5869..5887
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"