Basic Vector Information
- Vector Name:
- pcDNA3.1Zeo(-)-HBV1.1--C2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8240 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Wang W, Ni Y, Urban S.
- Promoter:
- EM7
pcDNA3.1Zeo(-)-HBV1.1--C2 vector Map
pcDNA3.1Zeo(-)-HBV1.1--C2 vector Sequence
LOCUS V015381 8240 bp DNA circular SYN 21-DEC-2019 DEFINITION Exported. ACCESSION V015381 VERSION V015381 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 8240) AUTHORS Wang W, Ni Y, Urban S. TITLE Direct Submission JOURNAL Submitted (03-NOV-2019) Molecular Virology, Department of Infectiology, Heidelberg University Hospital, Inf344, Heidelberg 69120, Deutschland REFERENCE 2 (bases 1 to 8240) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (03-NOV-2019) Molecular Virology, Department of Infectiology, Heidelberg University Hospital, Inf344, Heidelberg 69120, Deutschland" FEATURES Location/Qualifiers source 1..8240 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 235..614 /label="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 1324..3852 /gene="P" /label="Protein P" /note="Protein P from Hepatitis B virus genotype C subtype ad (isolate Japan/S-179/1988). Accession#: P03157" polyA_signal 4253..4477 /label="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 4523..4951 /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4965..5295 /label="SV40 promoter" /note="SV40 enhancer and early promoter" promoter 5343..5390 /label="EM7 promoter" /note="synthetic bacterial promoter" CDS 5409..5780 /label="BleoR" /note="antibiotic-binding protein" polyA_signal 5913..6046 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(6083..6099) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind complement(6107..6123) /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6131..6161) /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind complement(6176..6197) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6485..7073) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7247..8104) /label="AmpR" /note="beta-lactamase" promoter complement(8105..8209) /label="AmpR promoter"
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