pMEMORY8190 vector (V015360)

Download GeneBank File(.gb)

LOCUS       62056_16740        3958 bp DNA     circular SYN 06-SEP-2019
DEFINITION  Gateway destination vector pMEMORY8190 DNA, complete sequence.
ACCESSION   LC498629
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Fukuda E, Mori M, Shiku H, Miyahara Y, Kawamura Y, Ogawa K, Goshima 
            N.
  TITLE     Development of novel INSOL-tag enabling proteome-wide protein 
            handling and application to protein array
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Fukuda E, Goshima N.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2019) Contact:Eriko Fukuda National Institute of 
            Advanced Industrial Science and Technology, Molecular Profiling 
            Research Center for Drug Discovery; 2-4-7 Aomi, Koto-ku, Tokyo 
            135-0064, Japan
REFERENCE   3  (bases 1 to 3958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2019) Contact:Eriko Fukuda National Institute of Advanced 
            Industrial Science and Technology, Molecular Profiling Research 
            Center for Drug Discovery; 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, 
            Japan"
FEATURES             Location/Qualifiers
     source          1..3958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..58
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      183..193
                     /label=translation terminator
                     /note="translation terminator"
                     /regulatory_class="terminator"
     misc_feature    203..247
                     /label=mKG2-5C
                     /note="mKG2-5C"
     protein_bind    305..429
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1662..1786)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1806..1840
                     /label=translation terminator
                     /note="translation terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(2073..2661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2907..2940
                     /label=polyA
                     /note="polyA"
     CDS             complement(3013..3870)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3871..3941)
                     /label=AmpR promoter