pnnHispS-C1 vector (V015341)

LOCUS       62056_18170        9092 bp DNA     circular SYN 12-MAY-2020
DEFINITION  Cloning vector pnnHispS-C1, complete sequence.
ACCESSION   MT233537
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
  PUBMED    32341562
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic 
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 9092)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways, 
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     gene            603..5699
                     /gene="hispS"
                     /label=hispS
     CDS             603..5699
                     /codon_start=1
                     /transl_table=11
                     /gene="hispS"
                     /product="HispS"
                     /label=hispS
                     /note="derived from N. nambi"
                     /protein_id="QJQ48095.1"
                     /translation="MNSSKNPPSTLLDVFLDTARNLDTASRNVLECGEHRWSYRELDTV
                     SSALAQHLRYTVGLSPTVAVISENHPYILALMLAVWKLGGTFAPIDVHSPAELVAGMLN
                     IVSPSCLVIPSSDVTNQTLACDLNIPVVAFHPHQSTIPELNKKYLTDSQISPDLPFPDP
                     NRPALYLFTSSATSRSNLKCVPLTHTFILRNSLSKRAWCKRMRPETDFDGIRVLGWAPW
                     SHVLAHMQDIGPLTLLNAGCYVFATTPSTYPTELKDDRDVISCAANAVMYKGVKSFACL
                     PFVLGGLKALCESEPSVKAQLQVEERAQLLKSLQHMKILECGGAMLEVSVASWAIENRI
                     PISIGIGMTETGGALFAGPVQAIQTGFSSEDKFIEDATYLLVKDDYESHAEEDINEGEL
                     VVKSRMLPRGYLGYNDPSFSVDDAGWVTFKTGDRYSVTPDGKFSWLGRNTDFIQMTSGE
                     TLDPRPIESLLCESSLISRACVIGDKFLNGPATAVCAIIELEPTTVEKGQAHSRDIARI
                     FAPINRDLPPPLRIAWSHVLVLQPSEKIPMTKKGTIFRKKIEQVFGSALGGSSGDNSQA
                     TTDASVVRRDELSNTVKHIISRVLGVSDDELLWTLSFAELGMTSALATRIANELNEVLV
                     GVNLPINACYIHVDLPSLSNAVYAKLAHLKLPDRTPEPRKAPVENPGGKEIVIVGQAFR
                     LPGSINDVASLRDAFLARQASSIITEIPPDRWDHASFYPKDIRFNKAGLVDIANYDHSF
                     FGLTATEALYLSPTMRLALEVSFEALENANIPVSQLKGSQTAVYVATTDDGFETLLNAE
                     AGYDAYTRFYGTGRAASTASGRISYLLDVHGPSITVDTACSGGAVCIDQAIDYLQSSSA
                     ADTAIICASNTHCWPGSFMFLSAQGMVSSGGRCATFTTDADGYVPSEGAVAFILKTREA
                     AMRDKDTILATIKATQISHNGRSQGLVAPNVNSQADLHRSLLQKAGLSPADIHFIEAHG
                     TGTSLGDLSEIQAINDAYTSSQPRTAGPLIVSASKTVIGHTEPAGPLVGMLSVLNSFKE
                     GAVPGLAHLTADNLNPALDCSSVPLLIPYQPVHLAAPKPHRAAVMSYGFSGTLGGIVLE
                     APDEERLEEEPPNDKPMLFVVSAKTHTALIEYLGRYLEFLLQANPQDFCDICYTSCVGR
                     EHYRYRFACVANDMEDLIGQLQKRLGSKVPPKPSYKRGALAFAFSGQGTQFRGMATELA
                     KAYSGFRKIVSDLAKRASELSGHAIDRFLLAYDIGAENVAPDSEADQICIFVYQCSVLR
                     WLQTMGIRPSAVIGHSLGEISASVAAGALSLDSALDLVISRARLLRSSTNAPAGMAAMS
                     ASQDEVVELIGKLDLDKANSLSVSVINGPQNTVVSGSSAAIESIVALAKGRKIKASALN
                     INQAFHSPYVDSAVPGLRAWSEKHISSARPLQIPLYSTLLGAQVSEGQMLNPDHWVDHA
                     RKPVQFAQAATIMKESFTGVIIDIGPQVVAWSLLLSNGLTSVTALAAKRGRSQQVAFLS
                     ALADLYQDYGVVPDFVGLYAQQEDASRLKKTDILTYPFQRVRRYPSFIPSRRAPTHAHV
                     QDEETLSSGSSTPTLENTDLDSGKESLMGPTRGLLRVDDLRDSIVSSVKDVLELKSNED
                     LDLSESLNALGMDSIMFAQLRKRIGEGLGLSVPMVFLSDAFSIGEMVSNLVEQAEASED
                     N"
     misc_feature    5700..5756
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    5880..6001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6008..6463)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6490..6594
                     /label=AmpR promoter
     promoter        6596..6953
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6988..7779
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    8014..8061
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8390..8978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"