pTonBs-kanamycin-resistance-protein-gene vector (V015338)

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LOCUS       62056_21275        7597 bp DNA     circular SYN 24-FEB-2021
DEFINITION  Cloning vector pTonBs kanamycin resistance protein gene, complete 
            cds.
ACCESSION   MW132122
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition 
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University, 
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes 
            Ave, Pittsburgh, PA 15219-3016, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label=PnptII
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1916
                     /label=TonB dependent receptor 1 secretion signal
                     /note="TonB dependent receptor 1 secretion signal"
     misc_feature    1926..2150
                     /label=scorpine
                     /note="scorpine"
     misc_feature    2217..3554
                     /label=alkaline phosphatase, no leader signal
                     /note="alkaline phosphatase, no leader signal"
     CDS             complement(5146..5805)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5806..6575)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"