pFA-3xFLAG-MNase-CaHis1 vector (V015324)

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LOCUS       V015324                 4644 bp    DNA     circular SYN 30-SEP-2020
DEFINITION  Exported.
ACCESSION   V015324
VERSION     V015324
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Tebbji F, Khemiri I, Sellam A.
  TITLE     High resolution genome-wide occupancy in Candida spp. using ChEC-seq
  JOURNAL   bioRxiv (2020) In press
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Sellam S, Tebbji F, Khemiri I.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4644)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-MAR-2020) Microbiology, Infectious Disease "
FEATURES             Location/Qualifiers
     source          1..4644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..128
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     CDS             135..581
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             1180..2073
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     promoter        complement(2282..2300)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2558..3146)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3320..4177)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4178..4282)
                     /label="AmpR promoter"
     promoter        4628..4644
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"