pUC18T-miniTn7T-Zeo vector (V015308)

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LOCUS       62056_21975        4446 bp DNA     circular SYN 02-APR-2019
DEFINITION  Cloning vector pUC18T-miniTn7T-Zeo, complete sequence.
ACCESSION   MK356269
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Crisostomo L, Fernando DM, Chao TC, 
            Pelka P, Schweizer HP, Kumar A.
  TITLE     Next Generation of Tn7-based single copy insertion elements for use 
            in multi- and pandrug resistant strains of Acinetobacter baumannii
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
  PUBMED    30902859
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JAN-2019) Department of Microbiology, University of 
            Manitoba, 45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada
REFERENCE   3  (bases 1 to 4446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JAN-2019) Department of Microbiology, University of Manitoba, 
            45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada"
FEATURES             Location/Qualifiers
     source          1..4446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1118..1489)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(1508..1555)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(1734..1762)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1803..1850
                     /note="FRT; Flp recombinase target site"
     misc_feature    1877..1954
                     /note="MCS; multiple cloning site"
     mobile_element  complement(1963..2128)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(2287..2395)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(2627..3215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3389..4246)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4247..4351)
                     /label=AmpR promoter