Basic Vector Information
- Vector Name:
- 2-waxy-guide-CR1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3701 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Gao H.
2-waxy-guide-CR1 vector Map
2-waxy-guide-CR1 vector Sequence
LOCUS 62056_81 3701 bp DNA circular SYN 19-MAR-2020 DEFINITION Cloning vector 2-waxy-guide-CR1, complete sequence. ACCESSION MN294714 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3701) AUTHORS Gao H. TITLE Direct Submission JOURNAL Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA REFERENCE 2 (bases 1 to 3701) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA" COMMENT ##Assembly-Data-START## Assembly Method :: Vector NTI v. 10 Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3701 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 6..105 /label=attL1 /note="recombination site for the Gateway(R) LR reaction" regulatory 189..1188 /regulatory_class="promoter" misc_RNA 1209..1284 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" protein_bind complement(1356..1451) /label=attL3 /note="recombination site for the Gateway(R) LR reaction" promoter complement(1493..1511) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1516..1532) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 1645..2451 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 2601..3189 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(3519..3546) /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene"
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