pnnCPH-C1 vector (V015280)

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LOCUS       62056_18160        4916 bp DNA     circular SYN 12-MAY-2020
DEFINITION  Cloning vector pnnCPH-C1, complete sequence.
ACCESSION   MT233535
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
  PUBMED    32341562
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic 
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways, 
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     gene            603..1523
                     /gene="cph"
                     /label=cph
     CDS             603..1523
                     /codon_start=1
                     /transl_table=11
                     /gene="cph"
                     /product="CPH"
                     /label=cph
                     /note="derived from N. nambi"
                     /protein_id="QJQ48093.1"
                     /translation="MAPISSTWSRLIRFVAVETSLVHIGEPIDATMDVGLARREGKTIQ
                     AYEIIGSGSALDLSAQVSKNVLTVRELLMPLSREEIKTVRCLGLNYPVHATEANVAVPK
                     FPNLFYKPVTSLIGPGGLITIPSVVQPPKEHQSDYEAELVIVIGKAAKNVSEDEALDYV
                     LGYTAANDISFRKHQLAVSQWSFSKGFDGTNPLGPCLVSASSIPDPQDIPIQCKLNGGV
                     VQNGNTRDQIFNVKKTISFLSQGTTLEPGSIILTGTPDGVGFVRNPPLYLKDGDEVMTW
                     IGSGIGTLANTVREEQTCFASGGHE"
     misc_feature    1524..1580
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1704..1825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1832..2287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2314..2418
                     /label=AmpR promoter
     promoter        2420..2777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2812..3603
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3838..3885
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4214..4802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"