p15-Mev-aSFG vector (V015269)

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LOCUS       V015269                 8124 bp    DNA     circular SYN 11-FEB-2020
DEFINITION  Exported.
ACCESSION   V015269
VERSION     V015269
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Swift RP, Rajaram K, Liu HB, Dziedzic A, Jedlicka AE, Roberts AD,
            Matthews KA, Jhun H, Bumpus NN, Tewari SG, Wallqvist A, Prigge ST.
  TITLE     A mevalonate bypass system facilitates elucidation of plastid
            biology in malaria parasites
  JOURNAL   PLoS Pathog. (2020) In press
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Prigge ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2019) Molecular Microbiology and Immunology, Johns
            Hopkins SPH, 615 N. Wolfe, rm E5132, Johns Hopkins SPH, Baltimore,
            MD 21205, USA
REFERENCE   3  (bases 1 to 8124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-DEC-2019) Molecular Microbiology and Immunology, Johns Hopkins
            SPH, 615 N. Wolfe, rm E5132, Johns Hopkins SPH, Baltimore, MD 21205,
            USA"
FEATURES             Location/Qualifiers
     source          1..8124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     35..84
                     /label="AttP"
                     /note="AttP"
     3'UTR           91..919
                     /label="HSP86 3'"
                     /note="HSP86 3'"
     CDS             complement(941..967)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(974..1690)
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     mat_peptide     complement(1697..1864)
                     /gene="api-SFG-HA"
                     /product="HA-tagged Super Folder Green with an N-terminal
                     apicoplast trafficking peptide"
                     /note="Api55"
     5'UTR           1871..2837
                     /label="Pcam/Psti"
                     /note="Pcam/Psti"
     misc_feature    2844..3719
                     /gene="Mev"
                     /note="Region: Chimeric lower mevalonate pathway fusion
                     protein"
     CDS             3741..4316
                     /gene="PMVK"
                     /label="Phosphomevalonate kinase"
                     /note="Phosphomevalonate kinase from Homo sapiens.
                     Accession#: Q15126"
     CDS             4338..4883
                     /gene="idi"
                     /label="Isopentenyl-diphosphate Delta-isomerase"
                     /note="Isopentenyl-diphosphate Delta-isomerase from
                     Escherichia coli (strain K12 / DH10B). Accession#: B1XEH6"
     misc_feature    4905..5855
                     /gene="Mev"
                     /note="Region: Chimeric lower mevalonate pathway fusion
                     protein"
     3'UTR           5907..6469
                     /label="HRP2 3'"
                     /note="HRP2 3'"
     rep_origin      6594..7139
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        7183..7285
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             7286..7942
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"