Basic Vector Information
- Vector Name:
- pKK172
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9948 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Krawczyk K, Scheller L, Kim H, Fussenegger M.
- Promoter:
- EF-1α
pKK172 vector Map
pKK172 vector Sequence
LOCUS 62056_13460 9948 bp DNA circular SYN 15-FEB-2020 DEFINITION Expression vector pKK172, complete sequence. ACCESSION MN811106 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9948) AUTHORS Krawczyk K, Scheller L, Kim H, Fussenegger M. TITLE Rewiring of endogenous signaling pathways to genomic targets for therapeutic cell reprogramming JOURNAL Nat Commun 11 (1), 608 (2020) PUBMED 32001704 REFERENCE 2 (bases 1 to 9948) AUTHORS Krawczyk KK. TITLE Direct Submission JOURNAL Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, Switzerland REFERENCE 3 (bases 1 to 9948) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "608" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (09-DEC-2019) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, Switzerland" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..9948 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(324..548) /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" CDS complement(662..1378) /label=EGFP /note="enhanced GFP" CDS complement(2789..2815) /label=HA /note="HA (human influenza hemagglutinin) epitope tag" CDS complement(2822..2848) /label=HA /note="HA (human influenza hemagglutinin) epitope tag" CDS complement(2852..2878) /label=HA /note="HA (human influenza hemagglutinin) epitope tag" misc_feature complement(2894..2932) /gene="GEAR NFAT" /label=flexible linker /note="flexible linker" misc_feature complement(3924..4321) /gene="GEAR NFAT" /label=MCP /note="MCP" promoter complement(4353..5534) /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" CDS 6167..6868 /label=dTomato /note="dimeric variant of DsRed fluorescent protein (Shaner et al., 2004)" CDS 6935..7330 /label=BSD /note="blasticidin S deaminase" polyA_signal 7498..7553 /label=beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" rep_origin complement(8116..8704) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8878..9735) /label=AmpR /note="beta-lactamase" promoter complement(9736..9840) /label=AmpR promoter
This page is informational only.