pGLM177(P_EF1a-sigW) vector (V015259)

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LOCUS       V015259                 6286 bp    DNA     circular SYN 17-NOV-2020
DEFINITION  Exported.
ACCESSION   V015259
VERSION     V015259
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan GB, Khammash M,
            Siciliano V.
  TITLE     Characterization and mitigation of gene expression burden in
            mammalian cells
  JOURNAL   Nat Commun 11 (1), 4641 (2020)
   PUBMED   32934213
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Frei T, Cella F, Tedeschi F, Gutierrez J, Stan G-B., Khammash M,
            Siciliano V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2020) Synthetic and Systems Biology for
            Biomedicine, Italian Institute of Technology-IIT, Largo Barsanti e
            Martteucci 53, Naples, Campania 80125, Italy
REFERENCE   3  (bases 1 to 6286)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4641"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-AUG-2020) Synthetic and Systems Biology for Biomedicine, Italian
            Institute of Technology-IIT, Largo Barsanti e Martteucci 53, Naples,
            Campania 80125, Italy"
FEATURES             Location/Qualifiers
     source          1..6286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..266
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     polyA_signal    283..507
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        529..1710
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1741..2301
                     /gene="sigW"
                     /label="ECF RNA polymerase sigma factor SigW"
                     /note="ECF RNA polymerase sigma factor SigW from Bacillus
                     subtilis (strain 168). Accession#: Q45585"
     promoter        complement(2341..2359)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2517..2638
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      2728..3017
                     /note="misc_signal; INS"
                     /regulatory_class="other"
     rep_origin      complement(3096..3684)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3858..4715)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4716..4820)
                     /label="AmpR promoter"
     promoter        4890..5206
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5303..5899
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     regulatory      6132..6213
                     /label="SV40 pA"
                     /note="SV40 pA"
                     /regulatory_class="polyA_signal_sequence"