pEGFP-Msr1-m-6×His-Myc vector (V015018)

MSDS

Basic Vector Information

Vector Name:: pEGFP-Msr1-m-6×His-Myc

Antibiotic Resistance:: Kanamycin

Length:: 6135 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells

Selection Marker:: Neo/G418

Promoter:: CMV

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pEGFP-Msr1-m-6×His-Myc vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP-Msr1-m-6×His-Myc vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V015018                 6135 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015018
VERSION     V015018
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1342..2712
                     /gene="Msr1"
                     /label="Macrophage scavenger receptor types I and II"
                     /note="Macrophage scavenger receptor types I and II from
                     Mus musculus. Accession#: P30204"
     CDS             2722..2739
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    2923..3044
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3051..3506)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3533..3637
                     /label="AmpR promoter"
     promoter        3639..3996
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4031..4822
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5057..5104
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5433..6021
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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